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- Title
Propagation of CJD Prions in Primary Murine Glia Cells Expressing Human PrP c.
- Authors
Wälzlein, Joo-Hee; Schwenke, Karla A.; Beekes, Michael
- Abstract
There are various existing cell models for the propagation of animal prions. However, in vitro propagation of human prions has been a long-standing challenge. This study presents the establishment of a long-term primary murine glia culture expressing the human prion protein homozygous for methionine at codon 129, which allows in vitro propagation of Creutzfeldt–Jakob disease (CJD) prions (variant CJD (vCJD) and sporadic CJD (sCJD) type MM2). Prion propagation could be detected by Western blotting of pathological proteinase K-resistant prion protein (PrPSc) from 120 days post exposure. The accumulation of PrPSc could be intensified by adding a cationic lipid mixture to the infectious brain homogenate at the time of infection. Stable propagation of human prions in a long-term murine glia cell culture represents a new tool for future drug development and for mechanistic studies in the field of human prion biology. In addition, our cell model can reduce the need for bioassays with human prions and thereby contributes to further implementation of the 3R principles aiming at replacement, reduction and refinement of animal experiments.
- Subjects
PRIONS; CREUTZFELDT-Jakob disease; CATIONIC lipids; HUMAN biology; WESTERN immunoblotting; CELL culture
- Publication
Pathogens, 2021, Vol 10, Issue 8, p1060
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens10081060