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- Title
Comparison between Farr assay and BIOFLASH assay for identification of dsDNA-antibodies.
- Authors
McLean, Kyle; Vanderloo, Helen
- Abstract
Objectives: dsDNA antibodies are highly specific to systemic lupus erythroniums (SLE). The detection of dsDNA antibodies is used in the classification of SLE. The Farr assay is a radioimmunological assay used to detect high avidity IgG and IgM dsDNA antibodies and is considered the gold standard in dsDNA antibody detection. However, due to the radioactive component of the assay alternative assays have been considered, such as the Bio-Flash. The Bio-Flash assay detects only high avidity IgG dsDNA antibodies. However, IgM dsDNA antibodies are less clinically significant in the diagnosis of SLE. The purpose of this study was to compare the Bio-Flash assay to the Farr assay for identification of dsDNA antibodies. Methods: The dsDNA antibody test on the Bio-Flash is a chemiluminescent immunoassay. Calibration and quality control results were obtained prior to testing of patient samples. Sixty-nine patient samples were tested using the Farr assay, these samples were frozen upon completion and put aside to be tested using the Bio-Flash assay. Forty-nine of these were previously positive and 20 were previously negative based on the Farr assay results. The Bio-Flash results were collected and compared against the Farr assay results. Results: Of the 69 samples that were tested on the Bio-Flash a total of 20 discrepant results were collected. The correlation, frequency and inter-rater agreement of the results were compared. Conclusion: Due to the Bio-Flash results not being fully concordant with the Farr assay results in combination with the moderate correlation and agreeability of the assays, the BioFlash assay was found to be unreliable for standalone use. The Farr assay would need to remain in use along with the Bio-Flash assay if the assay were to be integrated.
- Subjects
RADIOIMMUNOASSAY; AUTOANTIBODIES; IMMUNOGLOBULINS; DNA; SYSTEMIC lupus erythematosus; CLINICAL pathology; CALIBRATION; INTER-observer reliability
- Publication
New Zealand Journal of Medical Laboratory Science, 2024, Vol 78, Issue 2, p101
- ISSN
1171-0195
- Publication type
Article