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- Title
Crystallization and preliminary X-ray diffraction analyses of the redox-controlled complex of terminal oxygenase and ferredoxin components in the Rieske nonhaem iron oxygenase carbazole 1,9a-dioxygenase.
- Authors
Matsuzawa, Jun; Aikawa, Hiroki; Umeda, Takashi; Ashikawa, Yuji; Suzuki-Minakuchi, Chiho; Kawano, Yoshiaki; Fujimoto, Zui; Okada, Kazunori; Yamane, Hisakazu; Nojiri, Hideaki
- Abstract
The initial reaction in bacterial carbazole degradation is catalyzed by carbazole 1,9a-dioxygenase, which consists of terminal oxygenase (Oxy), ferredoxin (Fd) and ferredoxin reductase components. The electron-transfer complex between reduced Oxy and oxidized Fd was crystallized at 293 K using the hanging-drop vapour-diffusion method with PEG 3350 as the precipitant under anaerobic conditions. The crystal diffracted to a maximum resolution of 2.25 Å and belonged to space group P21, with unit-cell parameters a = 97.3, b = 81.6, c = 116.2 Å, α = γ = 90, β = 100.1°. The VM value is 2.85 Å3 Da−1, indicating a solvent content of 56.8%.
- Subjects
OXYGENASES; FERREDOXINS; CRYSTALLIZATION; X-ray diffraction; CARBAZOLE; DIOXYGENASES; CHARGE exchange
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2014, Vol 70, Issue 10, p1406
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X14018779