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- Title
Matrix metalloproteinase -2, -8, -9, and -13 in gingival crevicular fluid of short root anomaly patients.
- Authors
Apajalahti, Satu; Sorsa, Timo; Ingman, Tuula
- Abstract
The aim of the present observational study was to identify and characterize matrix metallo- proteinase (MMP) -2, -8, -9, and -13 in gingival crevicular fluid (GCF) of patients with short root anomaly (SRA). GCF samples collected from affected maxillary central incisors and premolars of five SRA patients and five systemically and periodontally healthy controls were analysed using the zymographic technique for gelatinase A and B (MMP-2 and -9) and by Western blot for collagenase 2 and -3 (MMP-8 and -13). SRA GCF revealed MMP-9 (30 per cent of the total gelatinolytic activity), of which 18 per cent was in 90 kDa proform and 12 per cent in 71-82 kDa active form. Moreover, high molecular weight complexes (37 per cent) and low-molecular size fragmented (33 per cent) gelatlnolytlc enzymes were detectable. No MMP g or -13 immunoreactivities existed. These results may suggest that activation and complex formation of MMP-9 is characteristic of SRA GCF. From the findings it may be assumed that the GCF of SRA teeth has low collagenolytic resorptive or pathological activity.
- Subjects
METALLOPROTEINASES; GINGIVAL fluid; TOOTH root diseases; COLLAGENASES; SCIENTIFIC observation
- Publication
European Journal of Orthodontics, 2003, Vol 25, Issue 4, p365
- ISSN
0141-5387
- Publication type
Article
- DOI
10.1093/ejo/25.4.365