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- Title
Effects of Tumor Suppressor Gene TCF21 on the Proliferation, Migration and Apoptosis of A549 Cells.
- Authors
Song HU; Nuo YANG; Mingwu CHEN; Jianji GUO; Lei XIAN
- Abstract
Background and objective. TCF21, a newly discovered gene, exhibits tumor suppressor function in a variety of tumors. This study aims to observe the effects of TCF21 on the proliferation, apoptosis and migration of A549 human lung adenocarcinoma epithelial cells. Methods. TCF21 was overexpressed in A549 cells via lentiviral transfection. Fluorescence-based quantitative polymerase chain reaction and Western blot analysis were used to analyze the expression of the target gene. Transwell, proliferation assay, and flow cytometry were applied to detect the effect of TCF21 overexpression on the migration, proliferation, and apoptosis of A549 cells after transfection. Results. The proliferation and migration of A549 cells were inhibited, and the apoptotic rate was increased by overexpressing TCF21. Conclusion. The tumor suppressor gene, TCF21, significantly inhibits the proliferation and migration, as well as facilitates early apoptosis of A549 cells.
- Subjects
APOPTOSIS; CELL division; CELL lines; POLYMERASE chain reaction; RESEARCH funding; TUMOR necrosis factors; TUMORS; WESTERN immunoblotting; IMAGE cytometry
- Publication
Chinese Journal of Lung Cancer, 2014, Vol 17, Issue 4, p302
- ISSN
1009-3419
- Publication type
Article
- DOI
10.3779/j.issn.1009-3419.2014.04.03