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- Title
Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a.
- Authors
Teruel, Raúl; Martínez-Martínez, Irene; Guerrero, José A.; González-Conejero, Rocío; De la Morena-Barrio, María E.; Salloum-Asfar, Salam; Arroyo, Ana B.; Águila, Sonia; García-Barberá, Nuria; Miñano, Antonia; Vicente, Vicente; Corral, Javier; Martínez, Constantino
- Abstract
Background: Developmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant. Results: In this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control. Conclusions: These data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes.
- Subjects
ANTICOAGULANTS; PROTEIN synthesis; BIOMOLECULES; NEWBORN infants; GENETIC translation; MESSENGER RNA
- Publication
Journal of Biomedical Science, 2013, Vol 20, Issue 1, p1
- ISSN
1021-7770
- Publication type
Article
- DOI
10.1186/1423-0127-20-29