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- Title
Novel method for isolating human melanoblasts from keratinocyte culture.
- Authors
Cho, Eun ‐ Gyung; Bin, Bum ‐ Ho; Choi, Hyunjung; Park, Phil June; Kang, Hak Hee; Lee, Tae Ryong
- Abstract
The characterization of melanoblasts is important for understanding their in vivo development, melanoma formation, and pigment-related disorders. However, no methods have been reported for the isolation of melanoblasts from human skin. Using a 'calcium-pulse' technique, involving the differentiation of human keratinocytes with high calcium and the subsequent spontaneous separation of the epidermal sheets, we effectively isolated human melanoblasts (keratinocyte-adapted melanoblasts, Ka MBs) from keratinocyte culture. The Ka MBs expressed early melanogenesis-related genes, including BRN2, which is a known melanoblast marker. Moreover, the Ka MBs displayed much higher proliferative and growth capacities than the primary melanocytes. Considering that keratinocytes might provide an in vivo-like environment for Ka MBs during isolation and in vitro culture, the 'calcium-pulse' technique offers an unprecedented, easy, and efficient method for the isolation of human melanoblasts, retaining the original characteristics of these cells.
- Subjects
MELANOBLASTOMA; MELANOCYTES; PIGMENTATION disorders; MELANOGENESIS; EPIDERMAL growth factor receptor genetics; DISEASE risk factors
- Publication
Pigment Cell & Melanoma Research, 2014, Vol 27, Issue 3, p489
- ISSN
1755-1471
- Publication type
Article
- DOI
10.1111/pcmr.12221