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- Title
Cell-mediated cytotoxicity.
- Authors
de E. de Bracco, María M.; Isturiz, M. A.; Manni, J. A.
- Abstract
Isolated human mononuclear cells were fractionated according to their membrane characteristics or physical properties. Adherent cells were depleted by filtration through glass columns; phagocytic cells were removed by iron treatment and cell subpopulations capable of forming rosettes with sheep erythrocytes (E), erythrocyte-antibody-complement (EAC) and chicken erythrocyte-antibody complexes (CEA) were separated by centrifugation of Ficoll-Hypaque gradients. The functional activity of the cell subpopulations obtained was assayed by testing PHA-induced cytotoxicity (PIC), antibody- dependent cytotoxicity (ADCC) and blast transformation by PHA. The results of this study demonstrate that: (1) cells reacting in PIC and ADCC assays are different, adherent and phagocytic ceils being necessary for full expression of PIC and not for ADCC; (2) PHA induces direct blast transformation of purified E-RFC in the absence of PIC cytotoxic cells; (3) cell populations specifically enriched in E or EAC rosette-forming cells are not cytotoxic neither in the PHA nor in antibody mediated cytotoxic assays; (4) cells participating in ADCC can be selectively purified by centrifugation of CEA rosettes.
- Subjects
CELLS; ANTIBODY-dependent cell cytotoxicity; ERYTHROCYTES; CELLULAR immunity; KILLER cells; IMMUNOLOGY
- Publication
Immunology, 1976, Vol 30, Issue 3, p325
- ISSN
0019-2805
- Publication type
Article