We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Reinforcement of the Viral Safety of Hemoglobin Solution by Filtration and Heat Treatment.
- Authors
Abe, H.; Hirayama, J.; Sugawara, H.; Ikebuchi, K.; Tsuchida, E.; Ikeda, H.
- Abstract
Background: Stroma-free hemoglobin (SFH) is used as a source of artificial oxygen carrier. To increase the safety of SFH, nanofiltration and heat treatment were investigated as the methods of virus decontamination in SFH solution. Methods: Nonenveloped thermostable human parvovirus B19 (B19) and enveloped vesicular stomatitis virus (VSV) were used as model viruses. The SFH spiked with B19 was tangentially filtered using the cellulose-hollow fiber filter (BMM-15; mean pore size of 15 nm). The hemolysate spiked with VSV was treated at 60°C for 1 hr under either an air or CO atmosphere. Results: The BMM-15 filtration reduced B19 from 10[sup 8.3-8.7] PT (median PCR titer) to 10[sup 1.3-2.2] PT , indicating more than 6 log reduction. For the heat treatment, VSV was inactivated at >5.8 log and >6.0 log under the air and CO atmosphere, respectively. No additional methemoglobin formation was observed by the treatment under the CO atmosphere, despite the drastic methemoglobin formation under the air atmosphere. Alteration of hemoglobin banding was not observed by isoelectric focusing analysis in the sample after the heat treatment under the CO. Some protein bands other than hemoglobin were weakened or disappeared on SDS-PAGE after the heat treatment under both conditions. Conclusions: Enveloped viruses such as HIV can be inactivated by the heat treatment under the CO atmosphere without hemoglobin denaturation. Nonenveloped thermostable viruses such as B19 and hepatitis A virus can be removed by the nanofiltration. The combined use of the heat treatment and the nanofiltration will ensure the safety of SFH for red cell substitutes.
- Subjects
HEMOGLOBINS; BLOOD filtration; VIRUSES; INDUSTRIAL contamination
- Publication
Transfusion, 2001, Vol 41, p90S
- ISSN
0041-1132
- Publication type
Article