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- Title
Eavesdropping on PTM cross-talk through serial enrichment.
- Authors
Webb, Kristofor; Bennett, Eric J
- Abstract
The article cites a research study that examined the prevalence of phosphorylation and ubiquitylation occurring on the same protein extracted from actively replicating Saccharomyces cerevisiae cultures using two complementary approaches. The first approach used affinity purification of exogenous His-tagged ubiquitin, whereas the second distinguished which of the identified phosphorylation or ubiquitylation sites occurred on the same proteins.
- Subjects
PHOSPHORYLATION; UBIQUITIN; PROTEINS; SACCHAROMYCES cerevisiae; CHEMICAL reactions
- Publication
Nature Methods, 2013, Vol 10, Issue 7, p620
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2526