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- Title
Lipidic phase membrane protein serial femtosecond crystallography.
- Authors
Johansson, Linda C; Arnlund, David; White, Thomas A; Katona, Gergely; DePonte, Daniel P; Weierstall, Uwe; Doak, R Bruce; Shoeman, Robert L; Lomb, Lukas; Malmerberg, Erik; Davidsson, Jan; Nass, Karol; Liang, Mengning; Andreasson, Jakob; Aquila, Andrew; Bajt, Saša; Barthelmess, Miriam; Barty, Anton; Bogan, Michael J; Bostedt, Christoph
- Abstract
X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.
- Subjects
MEMBRANE proteins; CRYSTALLOIDS (Botany); FREE electron lasers; X-ray diffraction; FEMTOSECOND lasers; PHOTOSYNTHETIC reaction centers
- Publication
Nature Methods, 2012, Vol 9, Issue 3, p263
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1867