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- Title
Reevaluation of serum-plasma differences in total cholesterol concentration.
- Authors
Cloey, Teresa; Bachorik, Paul S.; Becker, Diane; Finney, Cheryl; Lowry, David; Sigmund, William; Cloey, T; Bachorik, P S; Becker, D; Finney, C; Lowry, D; Sigmund, W
- Abstract
We measured total cholesterol levels in serum and disodium ethylenediaminetetraacetate (EDTA) plasma samples obtained from 84 healthy medical students during their entrance physical examinations and 48 adults from a cholesterol screening program who were resampled because they had initial values of 5.17 mmol/L (200 mg/dL) or higher. Plasma cholesterol concentrations were 4.7% lower than those in serum samples. The plasma and serum values were highly correlated (r = .994), however, suggesting that measurements in EDTA plasma can be converted readily to equivalent serum concentrations. Thus, the negative bias in EDTA plasma was greater than the 3% value cited in the National Cholesterol Education Program Guidelines, probably because the amount of EDTA now provided in evacuated blood collection tubes is 50% greater than in those used when the 3% value was established. These findings are relevant to the interpretation of both cholesterol screening measurements and follow-up lipoprotein analyses.
- Subjects
BLOOD cholesterol; ETHYLENEDIAMINETETRAACETIC acid; BLOOD plasma; SERUM; CHOLESTEROL; COMPARATIVE studies; HEPARIN; RESEARCH methodology; MEDICAL cooperation; REGRESSION analysis; RESEARCH; EVALUATION research
- Publication
JAMA: Journal of the American Medical Association, 1990, Vol 263, Issue 20, p2788
- ISSN
0098-7484
- Publication type
journal article
- DOI
10.1001/jama.1990.03440200092029