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- Title
miR-144-5p Enhances the Radiosensitivity of Non-Small-Cell Lung Cancer Cells via Targeting ATF2.
- Authors
Song, Lei; Peng, Liping; Hua, Shucheng; Li, Xiaoping; Ma, Lianjun; Jie, Jing; Chen, Dong; Wang, Ying; Li, Dan
- Abstract
MicroRNAs (miRNAs or miRs) regulate gene expression at the posttranscriptional level and are involved in many biological processes such as cell proliferation and migration, stem cell differentiation, inflammation, and apoptosis. In particular, miR-144-3p is downregulated in various cancers, and its overexpression inhibits the proliferation and metastasis of cancer cells. However, the role of miR-144-5p in non-small-cell lung cancer (NSCLC), especially radiosensitivity, is unknown. In this study, we found that miR-144-5p was downregulated in NSCLC clinical specimens as well as NSCLC cell lines exposed to radiation. Enhanced expression of miR-144-5p promoted the radiosensitivity of NSCLC cells<italic> in vitro</italic> and A549 cell mouse xenografts<italic> in vivo</italic>. Furthermore, we identified activating transcription factor 2 (ATF2) as the direct and functional target of miR-144-5p using integrated bioinformatics analysis and a luciferase reporter assay. In addition, restoration of ATF2 expression inhibited miR-144-5p-induced NSCLC cell sensitivity to radiation<italic> in vitro</italic> and<italic> in vivo</italic>. Our findings suggest that deregulation of the miR-144-5p/ATF2 axis plays an important role in NSCLC cell radiosensitivity, thus representing a new potential therapeutic target for NSCLC.
- Subjects
RNA metabolism; CELL proliferation; ANIMAL experimentation; APOPTOSIS; BIOLOGICAL assay; CELL differentiation; CELL lines; GENE expression; LUNG cancer; MICE; TRANSCRIPTION factors; XENOGRAFTS; BIOINFORMATICS; IN vitro studies; IN vivo studies
- Publication
BioMed Research International, 2018, Vol 2018, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2018/5109497