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- Title
The effects of intravenous anesthetics on mouse embryonic fibroblast viability and proliferation.
- Authors
Choi, Yoon; Jeon, Se; Kim, Jungjoo; Park, Yongdoo; Yoon, Seung; Sun, Kyung
- Abstract
Purpose: The aim of this study is to evaluate the cytotoxic and antiproliferating effects of intravenous anesthetics on an mouse fibroblast in vitro cell culture system. Methods: The cells were exposed to the usual clinical plasma concentration of intravenous anesthetics, i.e., midazolam (0.15 μg/ml), propofol (2 μg/ml), remifentanil (2 μg/ml), thiopental (10 μg/ml), for 4, 8, or 24 h. Cell proliferation ( n = 6 for each) under intravenous anesthetics was analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Cytotoxicity ( n = 6 for each) of intravenous anesthetics was investigated using a LIVE/DEAD viability assay kit. Results: Intravenous anesthetic exposure time did not affect the proliferation rate of mouse fibroblasts. The cytotoxicity of intravenous anesthetics did not differ in accordance with exposure time. Conclusion: Our results showed that intravenous anesthetics may not affect mouse fibroblast proliferation and viability.
- Subjects
ANESTHETICS; FIBROBLASTS; CELL culture; CELL proliferation; MIDAZOLAM; WOUND healing
- Publication
Journal of Anesthesia, 2012, Vol 26, Issue 5, p675
- ISSN
0913-8668
- Publication type
Article
- DOI
10.1007/s00540-012-1427-1