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- Title
Arecoline‐regulated ataxia telangiectasia mutated expression level in oral cancer progression.
- Authors
Tu, Hsi‐Feng; Chen, Michael Yuanchien; Lai, Joseph Chieh‐Yui; Chen, Yi‐Ling; Wong, Yih‐Wen; Yang, Cheng‐Chieh; Chen, Hsin‐Yuan; Hsia, Shih‐Min; Shih, Yin‐Hwa; Shieh, Tzong‐Ming
- Abstract
Background: Ataxia telangiectasia mutated (ATM) regulates DNA repair and cell cycle. The present study analyzed arecoline‐induced ATM expression during oral cancer progression. Methods: In vitro studies were performed using oral squamous cell carcinoma (OSCC) cell lines treated with arecoline to analyze cell response and ATM regulation. in vivo studies were performed using immunohistochemistry to detect ATM expression in normal, oral potentially malignant disorder (OPMD), and OSCC tissues. Results: Low‐dose arecoline induced cell proliferation, ATM promoter activity, and DNA repair. High‐dose arecoline induced cell cycle arrest, apoptosis, and DNA damage. ATM was overexpressed in OPMD tissues but was downregulated in OSCC tissues. ATM expression level was associated with the risk of developing dysplasia, buccal‐OSCC, and with OSCC survival rate. Conclusion: High ATM expression helps DNA repair mechanisms to maintain the cells in the OPMD stage, but low ATM expression causes DNA damage accumulation to increase cell malignancy.
- Subjects
ATAXIA telangiectasia; ORAL cancer; CANCER invasiveness; DNA repair; SQUAMOUS cell carcinoma
- Publication
Head & Neck, 2019, Vol 41, Issue 8, p2525
- ISSN
1043-3074
- Publication type
Article
- DOI
10.1002/hed.25718