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- Title
Herpes Simplex Virus-1 qPCR in the Diagnosis of Lower Respiratory Tract Infections in Organ Transplant Recipients and Critically Ill Patients.
- Authors
Stram, Michelle N; Suciu, Christopher N; Seheult, Jansen N; McCullough, Melissa A; Kader, Muhamuda; Wells, Alan; Pasculle, A William; Rinaldo, Charles R; Ismail, Nahed; William Pasculle, A
- Abstract
<bold>Objectives: </bold>To determine a quantitative herpes simplex virus (HSV) DNA threshold in lower respiratory tract specimens that correlates with positive viral culture and clinical outcomes.<bold>Methods: </bold>Bronchoalveolar lavage and bronchial wash samples from 53 HSV culture-positive and 61 culture-negative matched controls were tested using HSV-1 and HSV-2 quantitative polymerase chain reaction (qPCR).<bold>Results: </bold>Median viral culture turnaround time was 21.8 days and 9.9 days for culture-negative and culture-positive specimens, respectively. Using an HSV-1 viral load threshold of 1.62 × 103 copies/mL, there was 93% agreement with viral culture. An HSV-1 viral load ≥1.3 × 104 copies/mL was associated with worse clinical outcome compared to a viral load <1.3 × 104 copies/mL (hazard ratio [HR] = 4.27, P = .017), and there was a trend of worse outcome compared to patients with undetectable HSV-1 DNA (HR = 1.60, P = .056).<bold>Conclusions: </bold>qPCR has clinical utility for rapid accurate identification of HSV-1 in lower respiratory tract specimens.
- Subjects
HERPES simplex virus; CRITICALLY ill; POLYMERASE chain reaction
- Publication
American Journal of Clinical Pathology, 2018, Vol 150, Issue 6, p522
- ISSN
0002-9173
- Publication type
journal article
- DOI
10.1093/ajcp/aqy088