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- Title
Evaluation of paroxysmal nocturnal hemoglobinuria screening by flow cytometry through multicentric interlaboratory comparison in four countries.
- Authors
Debliquis, Agathe; Wagner-Ballon, Orianne; Le Garff-Tavernier, Magali; Fossat, Chantal; Chatelain, Bernard; Letestu, Rémi; Drénou, Bernard; HPN-AFC Group
- Abstract
<bold>Objectives: </bold>Paroxysmal nocturnal hemoglobinuria (PNH) is currently diagnosed by flow cytometry; although highly sensitive, its interpretation and reporting appear as critical as its technique. Thus, we developed a quality control scheme for the French-speaking region based on the international recommendations for PNH screening.<bold>Methods: </bold>After a topical workshop, we proposed a 1-year, two-step survey program to any volunteering French-speaking clinical laboratory. The first survey consisted of sending raw data files to evaluate gating and the interpretation strategy of each center. The second stipulated sending fresh whole-blood samples to evaluate the whole process and its practice.<bold>Results: </bold>Forty-nine participants from voluntary centers returned results for each of the two successive surveys. On virtual survey, 27% reported false-positive PNH created by immature granulocytes, whereas the minor PNH clone was not detected by 9%. On fresh survey, 63% of centers used at least the same six-color combination (CD24, CD14, CD33, CD15, CD45, and fluorescent aerolysin), and nearly 70% of participants were able to perform a sensitivity test less than 0.1% on neutrophils. All participants detected the major PNH clone, yet 16% returned false-positive results for the non-PNH clone case.<bold>Conclusions: </bold>We succeeded in rallying numerous French-speaking clinical laboratories for both surveys and in harmonizing the technical practice by highlighting common pitfalls.
- Subjects
PAROXYSMAL hemoglobinuria; FLOW cytometry; AEROLYSIN; ROBUST statistics; BLOOD sampling; HEMOLYTIC anemia diagnosis; COMPARATIVE studies; DIAGNOSTIC errors; RESEARCH methodology; MEDICAL cooperation; RESEARCH; EVALUATION research; STANDARDS
- Publication
American Journal of Clinical Pathology, 2015, Vol 144, Issue 6, p858
- ISSN
0002-9173
- Publication type
journal article
- DOI
10.1309/AJCPWMWJ7XDNQ3SN