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- Title
The Utility of BRAF V600E Mutation-Specific Antibody VE1 for the Diagnosis of Hairy Cell Leukemia.
- Authors
Uppal, Guldeep; Ly, Vandi; Zi-Xuan Wang; Bajaj, Renu; Solomides, Charalambos C.; Banks, Peter M.; Medeiros, L. Jeffrey; Peiper, Stephen C.; Gong, Jerald Z.
- Abstract
Objectives: BRAF V600E mutation is characteristic of hairy cell leukemia (HCL). A V600E mutation-specific antibody, VE1, has been recently developed. We studied the diagnostic utility of this antibody in HCL and compared it with other B-cell neoplasms. Methods: VE1 activity was assessed using immunohistochemistry in 90 mature B-cell neoplasms, including HCL (n = 17), HCL variant (n = 6), chronic lymphocytic leukemia (CLL) (n = 20), and 47 other B-cell lymphomas. Most (87/90) specimens were formalin-fixed, paraffin-embedded bone marrow (BM) biopsy specimens decalcified in either hydrochloric acid or formic acid. Results: VE1 was positive in 15 (88%) cases of HCL and two (10%) cases of CLL and was negative in all other tumors assessed. The VE1-positive HCL cases showed uniform staining in all tumor cells, but intensity was variable. The two VE1-negative HCL cases had BRAF V600 mutations proven by molecular analysis. The two CLL cases positive with VE1 showed an atypical staining pattern with expression in a minority of lymphoma cells. Immunohistochemistry using the VE1 antibody had a sensitivity of 88% and a specificity of 97% for HCL. Conclusions: VE1 immunohistochemistry is a useful and convenient surrogate for detecting BRAF V600E mutation in BM biopsy specimens decalcified with hydrochloric or formic acid-based solutions.
- Subjects
BRAF genes; GENES; HAIRY cell leukemia; LEUKEMIA; LYMPHOPROLIFERATIVE disorders
- Publication
American Journal of Clinical Pathology, 2015, Vol 143, Issue 1, p120
- ISSN
0002-9173
- Publication type
Article
- DOI
10.1309/AJCPQLQ89VXTVWKN