We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Translational machinery of mitochondrial mRNA is promoted by physical activity in Western diet-induced obese mice.
- Authors
Lee, D. E.; Brown, J. L.; Rosa, M. E.; Brown, L. A.; Perry, R. A.; Washington, T. A.; Greene, N. P.
- Abstract
Aim Mitochondria-encoded proteins are necessary for oxidative phosphorylation; however, no report has examined how physical activity ( PA) and obesity affect mitochondrial mRNA translation machinery. Our purpose was to determine whether Western diet ( WD)-induced obesity and voluntary wheel running ( VWR) impact mitochondrial mRNA translation machinery and whether expression of this machinery is dictated by oxidative phenotype. Methods Obesity was induced with 8-wk WD feeding, and in the final 4 wks, half of mice were allowed VWR. Mitochondrial mRNA translation machinery including initiation factors (mt IF2/3), elongation factor Tu ( TUFM) and translational activator ( TACO1), and mitochondria-encoded proteins (CytB and ND4) was assessed by immunoblotting. The relation of mitochondrial mRNA translation to muscle oxidative phenotype was assessed using PGC-1 α transgenic overexpression ( MCK- PGC-1 α vs. wild-type mice) and comparing across muscle groups in wild-type mice. Results mt IF3 and TACO1 proteins were ~45% greater in VWR than sedentary ( SED), and TACO1 and mt IF2 proteins were ~60% and 125% greater in WD than normal chow ( NC). TUFM protein was ~50% lower in WD- SED than NC- SED, but ~50% greater in WD- VWR compared to NC- SED. CytB and ND4 were ~40% greater in VWR and ND4 was twofold greater with WD. TUFM, TACO1, ND4 and CytB were greater in MCK- PGC-1 α compared to wild-type, and mt IF2/3 contents were not different. In oxidative muscle (soleus), mitochondrial translation machinery was elevated compared to mixed (gastrocnemius) or glycolytic (extensor digitorum longus) muscles. Conclusion These data suggest a novel mechanism promoting mitochondrial function by translation of mitochondrial protein following PA. This may act to promote muscle health by PA in obesity.
- Subjects
OXIDATIVE phosphorylation; PHYSICAL activity; OBESITY; ELONGATION factors (Biochemistry); GENETIC overexpression
- Publication
Acta Physiologica, 2016, Vol 218, Issue 3, p167
- ISSN
1748-1708
- Publication type
Article
- DOI
10.1111/apha.12687