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- Title
The substrate specificity profile of human granzyme A.
- Authors
Van Damme, Petra; Maurer-Stroh, Sebastian; Hao, Han; Colaert, Niklaas; Timmerman, Evy; Eisenhaber, Frank; Vandekerckhove, Joël; Gevaert, Kris
- Abstract
The exact biological function of granzyme A, a granule-associated serine protease belonging to the tryptase family of proteases, is still a matter of debate because conflicting roles have been suggested, such as initiation of caspase-independent apoptosis-like cell death and endogenous modulation of inflammatory processes. In contrast to its well-studied family member, granzyme B, far less is known about the physiological targets of granzyme A. Using an N-terminal peptide-centric proteomics technology, the substrate specificity of human granzyme A was extensively characterized at the level of macromolecular protein substrates. Overall, more than 260 cleavage sites, almost exclusively favoring basic residues at the P1 position, in approximately 200 unique protein substrates, including the well-known in vitro substrates APEX-endonuclease 1 and different histones, were identified. Further substrate characterization was used to delineate physical properties in the substrate specificity profiles, which further highlights important aspects in protease/substrate biology.
- Subjects
CHROMATOGRAPHIC analysis; SERINE proteinases; PROTEOLYTIC enzymes; PROTEOMICS; PROTEINASES
- Publication
Biological Chemistry, 2010, Vol 391, Issue 8, p983
- ISSN
1431-6730
- Publication type
Article
- DOI
10.1515/BC.2010.096