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- Title
Effects of a monoclonal antibody against (pro)renin receptor on gliomagenesis.
- Authors
Fujimori, Takeshi; Shibayama, Yuki; Kanda, Takahiro; Suzuki, Kenta; Ogawa, Daisuke; Ishikawa, Ryou; Kadota, Kyuichi; Matsunaga, Toru; Tamiya, Takashi; Nishiyama, Akira; Miyake, Keisuke
- Abstract
Glioblastoma is characterized by a strong self-renewal potential and poor differentiated state. We have reported previously that the (pro)renin receptor [(P)RR] is a potential target for glioma therapy by silencing the (P)RR gene. Here, we have examined the effects of a monoclonal antibody against (P)RR on gliomagenesis. Human glioma cell lines (U251MG and U87MG) and a glioma stem cell line (MGG23) were used for the in vitro study. The expressions of the Wnt/β-catenin signaling pathway (Wnt signaling pathway) components and stemness markers were measured by Western blotting. The effects of the (P)RR antibody on cell proliferation, sphere formation, apoptosis and migration were also examined. Subcutaneous xenografts were also examined in nude mice. Treatment with the (P)RR antibody reduced expression of Wnt signaling pathway components and stemness markers. Furthermore, the (P)RR antibody reduced cell proliferation and decreased sphere formation significantly. The treatment also suppressed migration and induced apoptosis. In a subcutaneous xenograft model, systemic administration of the (P)RR antibody reduced tumor volume significantly. These data show that treatment with the (P)RR antibody is a potential therapeutic strategy for treating glioblastoma.
- Subjects
RENIN; STEM cells; CELLULAR signal transduction; CELL proliferation; WESTERN immunoblotting; PRORENIN receptor
- Publication
Scientific Reports, 2023, Vol 13, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-023-28133-x