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- Title
Differential early response of monocyte/macrophage subsets to intra-operative corticosteroid administration in lung transplantation.
- Authors
Glorion, Matthieu; Pascale, Florentina; Huriet, Maxime; Estephan, Jérôme; Gouin, Carla; Urien, Céline; Bourge, Mickael; Egidy, Giorgia; Richard, Christophe; Gelin, Valérie; De Wolf, Julien; Le Guen, Morgan; Magnan, Antoine; Roux, Antoine; Devillier, Philippe; Schwartz-Cornil, Isabelle; Sage, Edouard
- Abstract
Introduction: Lung transplantation often results in primary and/or chronic dysfunctions that are related to early perioperative innate allo-responses where myeloid subsets play a major role. Corticosteroids are administered upon surgery as a standard-of-care but their action on the different myeloid cell subsets in that context is not known. Methods: To address this issue, we used a cross-circulatory platform perfusing an extracorporeal lung coupled to cell mapping in the pig model, that enabled us to study the recruited cells in the allogeneic lung over 10 hours. Results: Myeloid cells, i.e. granulocytes and monocytic cells including classical CD14pos and non-classical/intermediate CD16pos cells, were the dominantly recruited subsets, with the latter upregulating the membrane expression of MHC class II and CD80/86 molecules. Whereas corticosteroids did not reduce the different cell subset recruitment, they potently dampened the MHC class II and CD80/86 expression on monocytic cells and not on alveolar macrophages. Besides, corticosteroids induced a temporary and partial anti-inflammatory gene profile depending on cytokines and monocyte/macrophage subsets. Discussion: This work documents the baseline effects of the standard-of-care corticosteroid treatment for early innate allo-responses. These insights will enable further optimization and improvement of lung transplantation outcomes.
- Subjects
LUNG transplantation; MACROPHAGES; MYELOID cells; ALVEOLAR macrophages; CORTICOSTEROIDS; STEM cell transplantation; HOMOGRAFTS
- Publication
Frontiers in Immunology, 2023, p01
- ISSN
1664-3224
- Publication type
Article
- DOI
10.3389/fimmu.2023.1281546