We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Effector and regulatory T cells derived from the same T cell clone differ in MHC class II-peptide multimer binding.
- Authors
Nolte-'t Hoen, Esther N. M.; Amoroso, Maria Grazia; Veenstra, Jetty; Grosfeld-Stulemeyer, Mayken C.; van Eden, Willem; Broeren, Chris P. M.; Wauben, Marca H. M.
- Abstract
MHC class II-peptide multimers are a valuable tool for antigen-specific detection of CD4 T cells. However, it has been proposed that T cells in a hypo-responsive state can have diminished binding of such multimers. In the present study, we investigated this phenomenon at the clonal level. We found that anergic CD4 T cells had a reduced capacity to bind MHC class II-peptide multimers compared to their non-anergic counterparts. Increasing the incubation temperature, time, or MHC-peptide valency could not equalize multimer binding by anergic and non-anergic T cells. Neither anergic T cells nor non-anergic T cells internalized the MHC class II-peptide dimers efficiently, and in both cases the dimers bound to the plasma membrane at locations containing a low amount of raft-associated lipids. Disruption of lipid rafts, however, led to decreased dimer binding by non-anergic T cells and to a lesser extent by anergic T cells. Finally, we show that the depth of the anergic state of the T cell, which determines its ability to regulate other T cell responses, correlates with the reduced dimer binding. We here demonstrate for the first time differential MHC class II-peptide multimer binding by regulatory (anergic) and effector T cells with identical TCR.
- Publication
European Journal of Immunology, 2004, Vol 34, Issue 12, p3359
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.200425563