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- Title
The Profile of Toll-like Receptor 2 (TLR2), TLR4 and Their Cytosolic Downstream Signaling Pathway in Common Variable Immunodeficiency (CVID) Patients.
- Authors
Sharifi, Laleh; Aghamohammadi, Asghar; Rezaei, Nima; yazdani, Reza; Mahmoudi, Mahdi; Amiri, Mohammad Mehdi; Masoumi, Farimah; Bokaie, Saied; Tavasolian, Parsova; Sanaei, Roozbeh; Moshiri, Mona; Tavakolinia, Naeimeh; Alinia, Tina; Azizi, Gholamreza; Mirshafiey, Abbas; Sanaei, Rouzbeh
- Abstract
Common variable immunodeficiency (CVID) is the most common clinical primary antibody deficiency, characterized by increased susceptibility to recurrent bacterial infections. Since Toll-like receptors (TLRs) play an important role in the maturation and differentiation of B-cells, TLRs' defect can be involved in the pathogenesis of CVID. Therefore, we evaluated the expression of TLR2 and TLR4 and their signaling pathway; also their association with autoimmunity, B-cell subtypes and response to pneumovax-23 were assessed in CVID patients. Sixteen CVID patients were enrolled in the study. Flow cytometry was used for assessing the protein expression of TLR2 and TLR4, and real-time PCR was used for gene expression of myeloid differentiation primary response 88 (MyD88) and toll interacting protein (Tollip). We found a higher protein expression of TLR2 in CVID patients which was associated with lower number of end stage B-cells and hyporesponse to pneumovax-23 vaccination. We showed a lower mRNA expression of MyD88 and an almost equal Tollip mRNA expression in CVID patients compared with controls. There was a profound association between MyD88 gene expression and autoimmunity in CVID patients. According to the presence of the lower number of end stage B-cells and poor vaccine response in CVID patients and their correlation with the higher expression of TLR2, we hypothesized that there is a functional defect in this receptor and/or its downstream in the peripheral blood mononuclear cells (PBMCs) of CVID patients.
- Subjects
TOLL-like receptors; JAK-STAT pathway; IMMUNODEFICIENCY; AUTOIMMUNITY; PROTEIN expression
- Publication
Iranian Journal of Allergy, Asthma & Immunology, 2018, Vol 17, Issue 2, p188
- ISSN
1735-1502
- Publication type
journal article