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- Title
Identification of Inhibitors of Phosphate-activated Glutaminase for the Pharmacotherapy of Schizophrenia.
- Authors
Sorrento, Cristina; Rayport, Stephen; Mihali, Andra; Kaunitz, Genevieve; Subramani, Shreya; Macdonald, Joanne; Katz, Francine
- Abstract
In the brain, phosphate-activated glutaminase catalyzes the recycling of glutamine back to glutamate for excitatory neurotransmission. In mice, genetic knockdown of phosphate-activated glutaminase has been shown to confer resilience to schizophrenia-like symptoms, suggesting that inhibition of glutaminase may have therapeutic potential for the pharmacotherapy of schizophrenia. As there are no known neuroactive inhibitors of glutaminase, i.e. inhibitors that get into the brain, high-throughput screening of a library of 58,000 neuroative compounds was conducted using a fluorescence-based assay; this screen identified 320 potential glutaminase inhibitors. A secondary screen was carried out to assess specificity; this yielded 10 hits. Using a kinetic analysis, two leads with low micromolar activity were found.
- Subjects
GLUTAMINASES; GLUTAMIC acid; NEURAL transmission; FLUORESCENCE microscopy; MICROBIOLOGICAL assay
- Publication
Columbia Undergraduate Science Journal, 2012, Vol 6, Issue 1, p3
- ISSN
1932-765X
- Publication type
Article