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- Title
Disulfide Bonds: A Key Modification in Bacterial Extracytoplasmic Proteins.
- Authors
Lee, S. F.; Davey, L.
- Abstract
Disulfide bonds are a common posttranslational modification that contributes to the folding and stability of extracytoplasmic proteins. Almost all organisms, from eukaryotes to prokaryotes, have evolved enzymes to make and break these bonds. Accurate and efficient disulfide bond formation can be vital for protein function; therefore, the enzymes that catalyze disulfide bond formation are involved in multiple biological processes. Recent advances clearly show that oral bacteria also have the ability to from disulfide bonds, and this ability has an effect on a range of dental plaque-related phenotypes. In the gram-positive Streptococcus gordonii, the ability to form disulfide bonds affected autolysis, extracellular DNA release, biofilm formation, genetic competence, and bacteriocin production. In Actinomyces oris, disulfide bond formation is needed for pilus assembly, coaggregation, and biofilm formation. In other gram-positive bacteria, such as Enterococcus faecalis, disulfide bonds are formed in secreted bacteriocins and required for activity. In these oral bacteria, the enzymes that catalyze the disulfide bonds are quite diverse and share little sequence homology, but all contain a CXXC catalytic active site motif and a conserved C-terminal cis-proline, signature features of a thiol-disulfide oxidoreductase. Emerging evidence also indicates that gram-negative oral bacteria, such as Porphyromonas gingivalis and Tannerella forsythia, use disulfide bonds to stabilize their outer membrane porin proteins. Bioinformatic screens reveal that these gram-negative bacteria carry genes coding for thiol-disulfide oxidoreductases in their genomes. In conclusion, disulfide bond formation in oral bacteria is an emerging field, and the ability to form disulfide bonds plays an important role in dental plaque formation and fitness for the bacteria.
- Subjects
DISULFIDES; CHEMICAL bonds; BACTERIAL cell walls; CYTOPLASM; OXIDOREDUCTASE genetics; BACTERIAL genetics; STREPTOCOCCUS gordonii; BACTERIOCIN genetics; BACTERIAL protein metabolism; BIOCHEMISTRY; DENTAL plaque; MATHEMATICAL models; PHENOMENOLOGY; MEMBRANE proteins; OXIDOREDUCTASES; SULFUR compounds; THEORY
- Publication
Journal of Dental Research, 2017, Vol 96, Issue 13, p1465
- ISSN
0022-0345
- Publication type
journal article
- DOI
10.1177/0022034517725059