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- Title
Putative stem cell markers in cervical squamous cell carcinoma are correlated with poor clinical outcome.
- Authors
Teng Hou; Weijing Zhang; Chongjie Tong; Kazobinka, Gallina; Xin Huang; Yongwen Huang; Yanna Zhang; Hou, Teng; Zhang, Weijing; Tong, Chongjie; Huang, Xin; Huang, Yongwen; Zhang, Yanna
- Abstract
<bold>Background: </bold>The aim of this study was to elucidate the value of putative cancer stem cell markers Musashi-1, ALDH1, Sox2, and CD49f in predicting the prognosis in cervical squamous cell carcinoma (CSCC).<bold>Methods: </bold>Real-time PCR and immunohistochemistry staining was performed to examine Musashi-1, ALDH1, Sox2, and CD49f expression in archived specimens of CSCC patients with postoperative chemotherapy. Kaplan-Meier analysis and Cox proportional hazards model were used to assess the prognostic impact of CSC markers for overall survival (OS) and recurrent-free survival (RFS).<bold>Results: </bold>The Real-time PCR data showed that the expression of all markers were increased in CSCC tissues compared with in paired normal cervical tissues (P < 0.05). The IHC result showed that high expression of Msi1, ALDH1, Sox2, and CD49f was found in 25.7%, 43.0%, 62.0% and 29.0% CSCC samples, respectively. Moreover, high expression of Msi1 (P = 0.033 and P = 0.003, respectively), ALDH1 (P = 0.015 and P = 0.002, respectively), and Sox2 (P = 0.005 and P = 0.003, respectively), and low expression of CD49f (P = 0.027 and P = 0.025, respectively) were correlated with poor OS and PFS in CSCC patients. Interestingly, tumors with Msi1(high)/CD49f(low) expression had the poorest prognosis according to Msi1/CD49f stratification. In multivariate Cox regression analysis, Sox2 expression (P = 0.047 and P = 0.018, respectively), ALDH1 expression (P = 0.013 and P = 0.003, respectively), and CD49f expression (P = 0.008 and P = 0.003, respectively) were independent prognostic markers for both OS and RFS.<bold>Conclusions: </bold>Our results suggest that cancer stem cell markers are linked with poor prognosis of CSCC patients.
- Subjects
CERVICAL cancer; BIOMARKERS; CANCER stem cells; SQUAMOUS cell carcinoma; CANCER chemotherapy; PROGNOSIS; ISOENZYMES; NERVE tissue proteins; PROTEINS; STEM cells; SURVIVAL; CERVIX uteri tumors; TREATMENT effectiveness; DIAGNOSIS
- Publication
BMC Cancer, 2015, Vol 15, p1
- ISSN
1471-2407
- Publication type
journal article
- DOI
10.1186/s12885-015-1826-4