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- Title
Overexpression of redox factor-1 negatively regulates NO synthesis and apoptosis in LPS-stimulated RAW 264.7 macrophages
- Authors
Yoo, Young Hyun; Lim, Young Jin; Park, Sang Eun; Kim, Jong-Min; Park, Young Chul
- Abstract
Redox factor-1 (Ref-1) is a ubiquitously expressed protein with proven roles as a modulator of redox-sensitive transcription, and as an endonuclease in the base excision repair pathway of oxidatively damaged DNA. Although Ref-1 is induced by a variety of oxidative stress and protects cells against oxidative stress, the function of Ref-1 in regulating nitric oxide (NO) synthesis has not been elucidated to date. We investigated the role of Ref-1 in regulating NO synthesis and NO-mediated apoptosis employing adenoviral-mediated overexpression of Ref-1 in lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. LPS treatment produced NO synthesis and NO-mediated apoptosis. Forced overexpression of Ref-1 suppressed LPS-stimulated NO synthesis. In parallel with this, Ref-1 also mitigated alteration of inducible NO synthase expression and NO-mediated apoptosis. Our findings suggest that Ref-1 is implicated in protection against cell death resulting from oxidative stimuli containing NO.
- Subjects
GENE expression; REACTIVE oxygen species; ENDONUCLEASES; OXIDATION-reduction reaction
- Publication
FEBS Letters, 2004, Vol 556, Issue 1-3, p39
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)01361-9