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- Title
A cDNA Clone of BcHSP81-4 from the Sterility Line (Pol CMS) of Non-heading Chinese Cabbage ( Brassica campestris ssp. chinensis).
- Authors
Liu, Tongkun; Hou, Xilin; Zhang, Jingyi; Song, Yuping; Zhang, Shuning; Li, Ying
- Abstract
BcHSP81-4 gene, a member of heat shock proteins, was identified from a suppression subtractive hybridization cDNA library in non-heading Chinese cabbage ( Brassica campestris ssp. chinensis Makino). The deduced amino acid sequence of the BcHSP81-4 cDNA revealed that it has high homology to other plant organelle isoforms and similar homology to both cytoplasmic and prokaryotic HSP90s. To study the regulation of gene expression, BcHSP81-4 genes in maintainer and sterility lines were monitored at different development stages and at different stress treatments. Real-time PCR was used for quantification of BcHSP81-4 mRNA. These results indicate that BcHSP81-4 is not responsive to heat shock at least at 35°C, while it is very responsive to salt and cold stress. And high expression of BcHSP81-4 in the bud of sterile line suggests that it may play prominent roles in sterility of pol CMS in non-heading Chinese cabbage.
- Subjects
CHINESE cabbage; ANTISENSE DNA; MOLECULAR cloning; HEAT shock proteins; AMINO acid sequence; MESSENGER RNA; GENE expression in plants; HOMOLOGY (Biology)
- Publication
Plant Molecular Biology Reporter, 2011, Vol 29, Issue 3, p723
- ISSN
0735-9640
- Publication type
Article
- DOI
10.1007/s11105-010-0285-y