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- Title
ThPOD3, a truncated polypeptide from <i>Tamarix hispida</i>, conferred drought tolerance in <i>Escherichia coli</i>.
- Authors
Xiao-Hong Guo; Jing Jiang; Bai-Chen Wang; Hui-Yu Li; Yu-Cheng Wang; Chuan-Ping Yang; Gui-Feng Liu
- Abstract
Abstract The ThPOD1 gene encodes a peroxidase and was isolated from a Tamarix hispida NaCl-stress root cDNA library. We found that ThPOD1 expression could be induced by abiotic stresses such as cold, salt, drought and exogenous abscisic acid. These findings suggested that ThPOD1 might be involved in the plant response to environmental stresses and ABA treatment. To elucidate the function of this gene, recombinant plasmids expressing full-length ThPOD1 as well as ThPOD2 (aa 41-337), and ThPOD3 (aa 73-337) truncated polypeptides were constructed. SDS–PAGE and Western blot analyses of the fusion proteins revealed that the molecular weights of ThPOD1, ThPOD2 and ThPOD3 were ~57, ~50 and ~47 kDa, respectively. Stress assays of E. coli treated with the recombinant plasmids indicated that ThPOD3 could improve resistance to drought stress. This finding could potentially be used to improve plant tolerance to drought stress via gene transfer.
- Subjects
DROUGHT tolerance; GENE fusion; MOLECULAR weights; GENETIC transformation; GENE expression; GENE expression in plants; ENVIRONMENTAL engineering; POLYPEPTIDES; TAMARISKS
- Publication
Molecular Biology Reports, 2010, Vol 37, Issue 3, p1183
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-009-9484-8