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- Title
延长马铃薯种质资源低温保存条件的探究.
- Authors
陈汝豪; 周艳华; 赵喜娟; 焦文标; 宋波涛; 柴婷婷
- Abstract
In the current potato germplasm resource preservation landscape, in vitro methods have gained widespread acceptance. Exploring the preservation conditions for extending the subculture cycle of test-tube seedlings can effectively reduce the risk and preservation cost. In this study, six different genotypes of potato materials were selected from the germplasm resources, and the effects of pre-rooting, temperature, sucrose concentration and subculture method on the preservation of potato test-tube seedlings were investigated using plant status, plant height and survival rate as indicators to explore the widely applicable conditions for long-term low temperature preservation of potato testtube seedlings. Test- tube seedlings without pre- rooting treatment showed minimal growth on the 60th day of low temperature preservation and eventually died, suggesting that pre- rooting treatment is essential for the successful low temperature preservation of test-tube seedlings. The growth rate of test-tube seedlings at 4℃ was slower compared to those at 7℃, and the plant height was obviously smaller on the 90th day. However, the survival rate of test-tube seedlings from four different materials at 7℃ was 100%, while it was only 53.75% at 4℃, indicating that a temperature of 7℃ is more suitable for preserving potato germplasm resources with various genotypes. Furthermore, the plant height of test- tube seedlings in a medium with 8% sucrose on the 90th day was obviously lower than those with 4% sucrose. Survival statistics revealed that all test-tube seedlings in the medium with 4% sucrose died after being stored at a temperature of 7℃ for 270 days, whereas there was still a survival rate of approximately 61.25% for those in an 8% sucrose concentration medium. These findings suggest that an 8% sucrose concentration could prolong the low temperature preservation time for potato test-tube seedlings. In short, using a sucrose concentration of 8% in combination with an ambient temperature of 7℃ could be effective for long-term low temperature preservation of most potato lines. Additionally, a subculture method involving test tube culture seeding and alternate box culture seedling was explored, which effectively improved the preservation of weak-growth germplasm resources and increased their survival rate from 22.50% (test tube culture seeding) to 95.00% (alternate subculture of test-tube culture seedlings-box culture seedlings) on the 90th day of low temperature preservation. This research provides valuable guidance for optimizing the conditions required for long-term preservation of potato germplasm resources.
- Publication
Chinese Potato Journal, 2023, Vol 37, Issue 5, p419
- ISSN
1672-3635
- Publication type
Article
- DOI
10.19918/j.cnki.1672-3635.2023.05.004