We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Leaves and Spiny Burs of Castanea Sativa from an Experimental Chestnut Grove: Metabolomic Analysis and Anti-Neuroinflammatory Activity.
- Authors
Chiocchio, Ilaria; Prata, Cecilia; Mandrone, Manuela; Ricciardiello, Fortuna; Marrazzo, Pasquale; Tomasi, Paola; Angeloni, Cristina; Fiorentini, Diana; Malaguti, Marco; Poli, Ferruccio; Hrelia, Silvana
- Abstract
Castanea sativa cultivation has been present in Mediterranean regions since ancient times. In order to promote a circular economy, it is of great importance to valorize chestnut groves' by-products. In this study, leaves and spiny burs from twenty-four Castanea trees were analyzed by 1H NMR metabolomics to provide an overview of their phytochemical profile. The Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) performed on these data allowed us to distinguish 'Marrone' from 'Castagna', since the latter were generally more enriched with secondary metabolites, in particular, flavonoids (astragalin, isorhamnetin glucoside, and myricitrin) were dominant. Knowing that microglia are involved in mediating the oxidative and inflammatory response of the central nervous system, the potential anti-inflammatory effects of extracts derived from leaves and spiny burs were evaluated in a neuroinflammatory cell model: BV-2 microglia cells. The tested extracts showed cytoprotective activity (at 0.1 and 0.5 mg/mL) after inflammation induction by 5 µg/mL lipopolysaccharide (LPS). In addition, the transcriptional levels of IL-1β, TNF-α, and NF-kB expression induced by LPS were significantly decreased by cell incubation with spiny burs and leaves extracts. Taken together, the obtained results are promising and represent an important step to encourage recycling and valorization of chestnut byproducts, usually considered "waste".
- Subjects
CHESTNUT; CASTANEA; MICROGLIA; LATENT structure analysis; INFLAMMATION; ORTHOGRAPHIC projection; LIPOPOLYSACCHARIDES
- Publication
Metabolites (2218-1989), 2020, Vol 10, Issue 10, p408
- ISSN
2218-1989
- Publication type
Article
- DOI
10.3390/metabo10100408