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- Title
Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum.
- Authors
Shi, Jing; Ma, Yuxin; Zheng, Meige; Ruan, Zhigang; Liu, Jing; Tian, Sumin; Zhang, Dexing; He, Xinhong; Li, Guoying
- Abstract
Occupational exposure and experimental intoxication of acrylamide (ACR) can produce skeletal muscle weakness and ataxia. In this study, we tested whether ACR would affect cerebellar function through the regulation of gamma-aminobutyric acid (GABA) and glial fibrillary acidic protein (GFAP) expression in cerebellum. Weaning male Sprague-Dawley rats were gavaged with ACR (5, 15, 30 mg/kg, 5 days per week) or saline for 4 weeks. Effects of ACR on the cerebellum were observed. For the 5 mg/kg group, no obvious change was observed, whereas moderate and severe ataxia were observed in the 15 mg/kg and 30 mg/kg groups, respectively. For the 15 mg/kg and 30 mg/kg groups, cerebellum concentrations of glutamate and GABA were dose-dependently decreased and increased, respectively. Moreover, the expression of GABA, the GABAergic presynaptic marker glutamate acid decarboxylase-65 (GAD65) and GFAP were significantly increased in those 2 groups. The results suggested that weaning rats were sensitive to ACR and that the toxic effects of ACR on the cerebellum may be associated with the increased expression of GABA and reactive astrocytes hypertrophy.
- Subjects
ACRYLAMIDE; ASTROCYTES; OCCUPATIONAL diseases; CEREBELLUM; SKELETAL muscle; AMINOBUTYRIC acid; LABORATORY rats
- Publication
Toxicology & Industrial Health, 2012, Vol 28, Issue 1, p10
- ISSN
0748-2337
- Publication type
Article
- DOI
10.1177/0748233711401264