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- Title
Granulocyte colony-stimulating factor enhances bone marrow mononuclear cell homing to the liver in a mouse model of acute hepatic injury.
- Authors
Shi-Zhu Jin; Xiang-Wei Meng; Xun Sun; Ming-Zi Han; Bing-Rong Liu; Xin-Hong Wang; Li-Ying Sun; Qi Huang; Rui-Bo Zhao; Xiang Ban; Hong-Ying Yu; Hong-Wei Yu; Jin, Shi-Zhu; Meng, Xiang-Wei; Sun, Xun; Han, Ming-Zi; Liu, Bing-Rong; Wang, Xin-Hong; Sun, Li-Ying; Huang, Qi
- Abstract
<bold>Background: </bold>Experiments have reported that granulocyte colony stimulating factor (G-CSF) can mobilize stem cells. However, few studies have examined the effect of G-CSF on bone marrow mononuclear cell (BMMC) mobilization, in particular regarding their capability to home to acutely injured liver.<bold>Aims: </bold>The aim of this study was to evaluate the effort of G-CSF on BMMC homing to the liver following chemically-induced hepatic failure.<bold>Methods: </bold>BMMC were isolated from mice, pre-labeled with PKH26 and infused into the mice in which hepatic injury had been induced followed by administration of G-CSF or vehicle. Livers were studied by fluorescent microscopy after transplantation of pre-labeled BMMC.<bold>Results: </bold>PKH26 labeled cells were found in liver tissue at 102 ± 10 cells/high power field in the BMMC+G-CSF group and 30 ± 5 cells/high power field in the BMMC group, but none in the G-CSF group and the control group (P < 0.05). In the former two groups the majority of PKH26 labeled cells colocalized with proliferative cell nuclear antigen (PCNA). The number of PCNA positive cells in the BMMC+G-CSF group was 20 ± 4 cells/high power field, while in the BMMC group it was 14 ± 2 cells/high power field, in the G-CSF group 12 ± 2 cells/high power field, and 8 ± 1 cells/high power field in the control group. Moreover, albumin expression was increased in the BMMC+G-CSF treated group (149 ± 7/high power field) relative to the BMMC group (48 ± 6/high power field), the G-CSF group (44 ± 5/high power field) and the vehicle group (30 ± 6/high power field), with the former three groups showing elevated levels as compared to vehicle control (30 ± 6) (P < 0.05).<bold>Conclusion: </bold>Transplanted BMMC may home to injured liver, which appears to be enhanced by G-CSF administration.
- Subjects
GRANULOCYTE-colony stimulating factor; STEM cells; BONE marrow; FLUORESCENCE microscopy; LIVER failure; LABORATORY mice; THERAPEUTICS; ANIMAL experimentation; ANIMALS; BIOLOGICAL models; BIOTHERAPY; BIOPSY; BONE marrow transplantation; CELLS; CELL motility; FLOW cytometry; HEMATOPOIETIC stem cells; HYDROCARBONS; IMMUNOHISTOCHEMISTRY; LIVER; MICE; ORGANIC compounds; ALBUMINS; ACUTE diseases; FLUORESCENT dyes
- Publication
Digestive Diseases & Sciences, 2010, Vol 55, Issue 10, p2805
- ISSN
0163-2116
- Publication type
journal article
- DOI
10.1007/s10620-009-1117-5