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- Title
Human Milk Oligosaccharides in Combination with Galacto- and Long-Chain Fructo-Oligosaccharides Enhance Vaccination Efficacy in a Murine Influenza Vaccination Model.
- Authors
Azarmi, Mehrdad; Seyed Toutounchi, Negisa; Hogenkamp, Astrid; Thijssen, Suzan; Overbeek, Saskia A.; Garssen, Johan; Folkerts, Gert; van't Land, Belinda; Braber, Saskia
- Abstract
Early-life nutrition significantly impacts vaccination efficacy in infants, whose immune response to vaccines is weaker compared to adults. This study investigated vaccination efficacy in female C57Bl/6JOlaHsd mice (6 weeks old) fed diets with 0.7% galacto-oligosaccharides (GOS)/long-chain fructo-oligosaccharides (lcFOS) (9:1), 0.3% human milk oligosaccharides (HMOS), or a combination (GFH) for 14 days prior to and during vaccination. Delayed-type hypersensitivity (DTH) was measured by assessing ear swelling following an intradermal challenge. Influvac-specific IgG1 and IgG2a levels were assessed using ELISAs, while splenic T and B lymphocytes were analyzed for frequency and activation via flow cytometry. Additionally, cytokine production was evaluated using murine splenocytes co-cultured with influenza-loaded dendritic cells. Mice on the GFH diet showed a significantly enhanced DTH response (p < 0.05), increased serological IgG1 levels, and a significant rise in memory B lymphocytes (CD27+ B220+ CD19+). GFH-fed mice also exhibited more activated splenic Th1 cells (CD69+ CXCR3+ CD4+) and higher IFN-γ production after ex vivo restimulation (p < 0.05). These findings suggest that GOS/lcFOS and HMOS, particularly in combination, enhance vaccine responses by improving memory B cells, IgG production, and Th1 cell activation, supporting the potential use of these prebiotics in infant formula for better early-life immune development.
- Subjects
INFLUENZA prevention; IMMUNOGLOBULIN analysis; FLOW cytometry; RESEARCH funding; REGULATORY B cells; VACCINE effectiveness; INFLUENZA vaccines; ENZYME-linked immunosorbent assay; PREBIOTICS; BREAST milk; DESCRIPTIVE statistics; INFANT nutrition; MICE; ANIMAL experimentation; CYTOKINES; OLIGOSACCHARIDES; EVALUATION
- Publication
Nutrients, 2024, Vol 16, Issue 17, p2858
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu16172858