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- Title
Interleukin-3 and Interleukin-3 Receptors in the Brain<sup>a</sup>.
- Authors
TABIRA, TAKESHI; CHUI, DE-HUA; FAN, JI-PING; SHIRABE, TERUO; KONISHI, YOSHIHIRO
- Abstract
We have previously demonstrated that interleukin 3 (IL-3) has a neurotrophic effect on central cholinergic neurons and have demonstrated the presence of IL-3 receptor (IL-3R)β subunits in septal cholinergic neurons by reverse-transcribed polymerase chain reaction (RT-PCR) and immunohistochemistry. In order to confirm that the expressed IL-3R is functional, we conducted experiments to show an a subunit of IL-3R. The α subunit was clearly demonstrated by RT-PCR in the central cholinergic neuronal hybrid cell line SN6, but not in its mother cell line N18TG2, and the expression was slightly upregulated after IL-3 treatment. Choline acetyltransferase and vesicular acetylcholine transporter mRNAs were significantly increased in SN6 after treatment with IL-3. Immunohistochemically, IL-3Rα-positive cells were mainly present in the medial septal and basal forebrain region, and the stained cells were similar to choline acetyltransferase-positive cells in shape and distribution. The IL-3Rα-positive cells slightly increased two days after fimbria-fornix transection and decreased seven days after. These findings suggest that functional IL-3 receptors are expressed in the central cholinergic neurons and contribute to some physiological roles such as the differentiation and maintenance of these neurons.
- Publication
Annals of the New York Academy of Sciences, 1998, Vol 840, Issue 1, p107
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1111/j.1749-6632.1998.tb09554.x