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- Title
Microbial Contamination in Shaker Bottles among Members of Fitness Centers.
- Authors
Silveira, Matheus Baffi; Scudese, Estevão; Senna, Gilmar Weber; Ferreira, Ana Paula; Dantas, Estélio Henrique Martin; a, Luiz Cláudio Pereira; Alvares, Alessandra Fiuza Hoelz; Garcia, Patrícia Guedes
- Abstract
The purpose of this was to analyze the presence of different bacterial strains and to determine resistance to antimicrobials in used bottles (UB) from the members of fitness centers and new non-used bottles (NUB). A total of 60 shakers (30 UB and 30 NUB) were selected and submitted to microbiological analysis. The samples were collected through Swabs containing Stuart's medium and delivered to the laboratory. Gram staining and biochemical tests were performed for the identification of microorganisms and antimicrobial susceptibility. The Cochran Q test presented significant difference (P=0.001) in contamination status (UB vs. NUB). All the NUB tests showed 100% absence of contamination while 90% of the UB showed bacterial contamination. For the 60 samples investigated, we were able to isolate six species of different microorganisms. It is important to note that 16.6% had no bacterial growth. The antibacterial susceptibility test revealed a varied range of resistance profile. In conclusion, six pathogenic microorganisms were isolated from poorly sanitized bottles highlighting that the post-use hygiene must be made appropriately to avoid the proliferation of pathogenic bacteria.
- Subjects
MICROBIAL contamination; PHYSICAL fitness centers; GRAM'S stain; PATHOGENIC bacteria; EXERCISE physiology; PHYSICAL fitness
- Publication
Journal of Exercise Physiology Online, 2018, Vol 21, Issue 4, p134
- ISSN
1097-9751
- Publication type
Article