We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
MiR-125b regulates the proliferation and metastasis of triple negative breast cancer cells via the Wnt/β-catenin pathway and EMT.
- Authors
Nie, Jun; Jiang, Hong-Chao; Zhou, Yong-Chun; Jiang, Bo; He, Wen-Jie; Wang, Yu-Feng; Dong, Jian
- Abstract
Background/aim: MiR-125b plays an important role in breast cancer. The current study was to explore the expression and function of miR-125b in triple negative breast cancer cells. Materials and methods: The expression of miR-125b in human TNBC samples and cell lines were examined by qRT-PCR. MTT, scratch assays and transwell assays were utilized to observe the proliferation, migration and invasion ability. MiR-125b's target gene and downstream signaling pathways were investigated by Luciferase Reporter Assays, qRT-PCR, immunofluorescence assays and western bolt. Results: MiR-125b was highly expressed in human TNBC tissues and cell lines. Inhibiting miR-125b expression suppressed the proliferation, cell migration and invasion. The three-prime untranslated region (3´-UTR) of adenomatous polyposis coli (APC) mRNA contains miR-125b binding sites, and inhibiting miR-125b expression suppressed the activity of the intracellular Wnt/β-catenin pathways and EMT. Conclusion: Inhibiting miR-125b regulates the Wnt/β-catenin pathway and EMT to suppress the proliferation and migration of MDA-MB-468 TNBC cells. MiR-125b was highly expressed in human TNBC tissues and cell lines. Inhibiting miR-125b expression suppressed the proliferation, cell migration and invasion of MDA-MB-468 TNBC cells by targeting adenomatosis polyposis coli (APC) to regulate the Wnt/β-catenin pathway and EMT.
- Subjects
CELL proliferation; METASTASIS; CATENINS
- Publication
Bioscience, Biotechnology & Biochemistry, 2019, Vol 83, Issue 6, p1062
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2019.1584521