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- Title
Electrochemical DNA sensing based on gold nanoparticle amplification.
- Authors
Zhi-Ling Zhang; Dai-Wen Pang; Hong Yuan; Ru-Xiu Cai; Abruña, Héctor D.
- Abstract
A hybridization signal-amplified method based on a gold nanoparticle-supported DNA sequence for electrochemical DNA sensing has been investigated by cyclic voltammetry, differential-pulse voltammetry, and atomic-force microscopy (AFM). Quantitative analysis showed that the peak current increment (?Ip) is linearly dependant on the concentration of the gold nanoparticle-supported DNA sequenceAu2over the range 0.51-8.58 pmol L-1. AFM results indicated that the extent of surface hybridization was dependent on the concentration of the gold-nanoparticle-supported DNA sequence. Moreover, a new pair of peaks, which might arise from the special configuration of the gold-nanoparticle-supported DNA sequence, appeared in the cyclic voltammogram after hybridization. Although quite sensitive, this DNA sensing surface was not easily regenerated, so this kind of amplified method was suitable for disposable DNA sensors and chip-based gene diagnosis sensors.
- Subjects
NUCLEOTIDE sequence; DNA; NANOPARTICLES; ATOMIC force microscopy; DETECTORS
- Publication
Analytical & Bioanalytical Chemistry, 2005, Vol 381, Issue 4, p833
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-004-2972-8