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- Title
Studying Intrinsically Disordered Proteins under True In Vivo Conditions by Combined Cross-Polarization and Carbonyl-Detection NMR Spectroscopy.
- Authors
Lopez, Juan; Schneider, Robert; Cantrelle, Francois‐Xavier; Huvent, Isabelle; Lippens, Guy
- Abstract
Under physiological conditions, studies of intrinsically disordered proteins (IDPs) by conventional NMR methods based on proton detection are severely limited by fast amide-proton exchange with water. 13C detection has been proposed as a solution to the exchange problem, but is hampered by low sensitivity. We propose a new pulse sequence combining proton-nitrogen cross-polarization and carbonyl detection to record high-resolution, high-sensitivity NMR spectra of IDPs under physiological conditions. To demonstrate the efficacy of this approach, we recorded a high-quality N-CO correlation spectrum of α-synuclein in bacterial cells at 37 °C.
- Subjects
PROTEIN research; PROTON detection; PROTEIN structure; NUCLEAR magnetic resonance spectroscopy; MICROBIAL sensitivity tests
- Publication
Angewandte Chemie, 2016, Vol 128, Issue 26, p7544
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201601850