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- Title
Enzymatically Shifting Nitroxides for EPR Spectroscopy and Overhauser-Enhanced Magnetic Resonance Imaging.
- Authors
Audran, Gérard; Bosco, Lionel; Brémond, Paul; Franconi, Jean‐Michel; Koonjoo, Neha; Marque, Sylvain R. A.; Massot, Philippe; Mellet, Philippe; Parzy, Elodie; Thiaudière, Eric
- Abstract
In vivo investigations of enzymatic processes using non-invasive approaches are a long-lasting challenge. Recently, we showed that Overhauser-enhanced MRI is suitable to such a purpose. A β-phosphorylated nitroxide substrate prototype exhibiting keto-enol equilibrium upon enzymatic activity has been prepared. Upon enzymatic hydrolysis, a large variation of the phosphorus hyperfine coupling constant (Δ aP=4 G) was observed. The enzymatic activities of several enzymes were conveniently monitored by electronic paramagnetic resonance (EPR). Using a 0.2 T MRI machine, in vitro and in vivo OMRI experiments were successfully performed, affording a 1200 % enhanced MRI signal in vitro, and a 600 % enhanced signal in vivo. These results highlight the enhanced imaging potential of these nitroxides upon specific enzymatic substrate-to-product conversion.
- Subjects
NITROXIDES; ELECTRON paramagnetic resonance spectroscopy; MAGNETIC resonance imaging; DYNAMIC nuclear polarisation; CHEMICAL research
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 45, p13577
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201506267