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- Title
Improved substrate specificity of water-soluble pyrroloquinoline quinone glucose dehydrogenase by a peptide ligand.
- Authors
Yoshida, Hiromi; Yagi, Yukiko; Ikebukuro, Kazunori; Sode, Koji
- Abstract
A new approach in altering the substrate specificity of enzyme is proposed using glucose dehydrogenase, with pyrroroquinoine quinone (PQQGDH) as co-factor, as the model. This approach is based on the selection of random peptide phage displayed library. Using an M13 phage-display random peptide library, we have selected peptide ligands. Among the peptide ligands, a 7-mer peptide, composed of Thr-Thr-Ala-Thr-Glu-Tyr-Ser, caused PQQGDH substrate specificity to decrease significantly toward disaccharides, such as maltose and lactose, while a smaller effect was observed toward glucose. Consequently, this peptide narrowed the substrate specificity of PQQGDH, without a significant loss of the enzyme activity.
- Subjects
PQQ (Biochemistry); DEHYDROGENASES; GLUCOSE; PEPTIDES; LIGANDS (Biochemistry); DISACCHARIDES
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 4, p301
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1022345200666