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- Title
Improvement of protein stability in protein microarrays.
- Authors
Lee, Chang-Soo; Kim, Byung-Gee
- Abstract
Protein stability in microarrays was improved using protein stabilizers. PEG 200 at 30% (w/v) was the most efficient stabilizer giving over 4-fold improvement in protein stability compared to without the stabilizer. PEG 200 above 10% (w/v) in the array solution prevented the evaporation of water in the sample and thereby improved protein stability in the microarray. When the streptavidin-biotin binding reaction was performed under optimized conditions, biotin-BSA-fluorescein isothiocyanate (FITC) was detected from 1 ng ml-1 to 5 μg ml-1 by fluorescence analysis.
- Subjects
PROTEIN microarrays; IMMOBILIZED proteins; BIOCHIPS; BACTERIAL proteins; MICROBIAL proteins; VITAMIN B complex
- Publication
Biotechnology Letters, 2002, Vol 24, Issue 10, p839
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1015554705979