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- Title
Production of vitamin B12 in an upflow anaerobic filter continuous reactor using Acetobacterium sp.
- Authors
Bainotti, Alberto Emilio; Estebanez, Belén; Nagadomi, Hisashi; Nishio, Naomichi
- Abstract
The accumulation of biofilm by Acetobacterium sp. during continuous culture in an upflow anaerobic filter (UAF) growing on methanol-formate was the result of space velocity and inlet concentrations of substrate and Co+2. To achieve good development of biofilm, a space velocity of 0.38 h-1, inlet substrate concentrations of 125 mM of both methanol and formate, and Co+2 at 0.16 mM were required. Cell productivities in the effluent of the UAF-reactor were about 6-fold higher than in chemostat cultures (0.20 g l-1 h-1 for UAF and 0.035 g l-1 h-1 for chemostat) (previous studies), and the maximum vitamin B12 specific concentration was 5.1 mg g cell-1.
- Subjects
ANAEROBIC bacteria; VITAMIN B12; VITAMIN B complex; METHANOL; BIOFILMS; MICROBIAL aggregation
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 6, p503
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005612819257