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- Title
Partitioning of bovine lactoferrin in aqueous two-phase system containing poly(ethylene glycol) and sodium citrate.
- Authors
da Costa, Angélica Ribeiro; dos Reis Coimbra, Jane Sélia; Ferreira, Luísa Alexandre; Marcos, João Carlos; Santos, Igor José Boggione; Saldaña, Marleny D. A.; Couto Teixeira, José António
- Abstract
The partitioning of the whey protein lactoferrin, which is an iron transporter glycoprotein, in an aqueous two-phase system composed of poly(ethylene glycol) (PEG) and sodium citrate was evaluated. Equilibrium data at 25 °C were determined for each system studied using PEG with a molar mass of 1000 and 4000 g mol-1 at pH values of 5.5, 6.5, and 7.5. An increase in the molar mass of the polymer promoted the expansion of the two-phase region and caused the migration of the lactoferrin to the salt-rich bottom phase. An increase in pH also led to the expansion of the biphasic region. However, changing the pH over the tested range slightly affected protein partitioning. Lactoferrin recovery percentages greater than 94% were observed for all of the systems evaluated. The results indicated that lactoferrin can be successfully partitioned in an aqueous two-phase system formed of 14% (w/w) PEG and 10% (w/w) sodium citrate at pH 5.5 and 25 °C. The protein was concentrated 1000-fold in the salt-rich bottom phase in this system.
- Subjects
BOVINE anatomy; LACTOFERRIN; GLYCOPROTEINS; MOLAR mass; CARRIER proteins
- Publication
Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C, 2015, Vol 95, p118
- ISSN
0960-3085
- Publication type
Article
- DOI
10.1016/j.fbp.2015.04.002