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- Title
miR-498 promotes cell proliferation and inhibits cell apoptosis in retinoblastoma by directly targeting CCPG1.
- Authors
Yang, Ling; Wang, Li; Wang, Xiang; Liu, Qing-Huai; Wei, Ning
- Abstract
Purpose: Retinoblastoma (Rb) is the most common intraocular tumor in children. MicroRNAs (miRNAs) play a crucial role in gene regulation and cell growth/apoptosis/differentiation. The current study aimed to investigate the role of miR-498 in Rb.Methods: Quantitative real-time polymerase chain reaction (QRT-PCR) was used to test mRNA level of miR-498. <ext-link>http://www.targetscan.org</ext-link> and <ext-link>http://www.microrna.org</ext-link> were applied to predict target of miR-498. Dual-luciferase reporter assay was applied to investigate if miR-498 targeted cell cycle progression 1 (CCPG1). Western blot (WB) was carried out to assess CCPG1 protein levels. 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay was used to evaluate cell proliferation. Annexin-V Fluorescein (FITC) was adopted to explore cell apoptosis.Results: In Y79 cells, miR-498 was higher than in normal ARPE-19 cells. MiR-498 could recognize CCPG1-3′ untranslated region (UTR). CCPG1 protein level was remarkably decreased when overexpressed miR-498, nevertheless, significantly increased when inhibiting miR-498. Y79 cells that were transfected with miR-498 mimics manifested notable cell apoptosis down-regulation and cell proliferation promotion; whereas, those transfected with miR-498 inhibitor displayed significant cell apoptosis up-regulation and cell proliferation inhibition compared with control group.Conclusion: Taken together, miR-498 promotes cell proliferation and inhibits cell apoptosis in Rb by directly targeting CCPG1.
- Subjects
RETINOBLASTOMA; CELL proliferation; MICRORNA; POLYMERASE chain reaction; CELL cycle; THERAPEUTICS
- Publication
Child's Nervous System, 2018, Vol 34, Issue 3, p417
- ISSN
0256-7040
- Publication type
Article
- DOI
10.1007/s00381-017-3622-8