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- Title
Juvenile hormone mediates the positive effects of nitrogen fertilization on weight and reproduction in pea aphid.
- Authors
Guo, Hui Juan; Sun, Yu Cheng; Gao, Jing; Ge, Feng
- Abstract
BACKGROUND: The positive effects of nitrogen fertilization on the performance of phytophagous insects have been reported extensively; the physiological and molecular basis involved, however, is largely unclear. Here, we test experimentally whether enhancement of juvenile hormone (JH) is responsible for the increased weight and fecundity of pea aphid (Acyrthosiphon pisum) under nitrogen fertilization. RESULTS: Aphids fed on Medicago truncatula with nitrogen fertilization have a greater amino acid content, higher weight at the fourth instar and adult stage, and produce more offspring than those without nitrogen fertilization. Furthermore, nitrogen fertilization upregulates the transcripts of JH biosynthesis‐related genes and increases JH titre at the fourth instar and adult stage, suggesting that JH is involved in the positive responses of aphids to nitrogen fertilization. Application of 100 ng JH increases adult weight and fecundity in aphids fed on M. truncatula without nitrogen fertilization. Conversely, impairing JH signalling by pharmacologically inhibiting the target of rapamycin pathway or by knocking down JH biosynthetic gene decreases adult weight and fecundity in aphids fed on M. truncatula with nitrogen fertilization, whereas application of JH rescued the phenotype. CONCLUSION: The increased JH titre at the fourth instar and adult stage is required for the increases of weight and fecundity of A. pisum under nitrogen fertilization. © 2018 Society of Chemical Industry Increased amino acid contents enhanced juvenile hormone biosynthesis of pea aphids at fourth instar and adult stage, which is responsible for the enhanced weight and fecundity under nitrogen fertilization.
- Subjects
PEA aphid; AMINO acids; MEDICAGO truncatula; NITROGEN fertilizers; FERTILITY
- Publication
Pest Management Science, 2018, Vol 74, Issue 11, p2511
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.4932