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- Title
Ethapolan synthesis by Acinetobacter sp. IMV B-7005 on the mixture of C2-C6-substrates and waste sunflower oil.
- Authors
Voronenko, Andrii; Pirog, Tetyana
- Abstract
Introduction. The cultivation conditions of Acinetobacter sp. IMV B-7005, which would provide maximum synthesis of the microbial exopolysaccharide (EPS) ethapolan on the mixture of C2- C6-substrates (molasses, acetate, or ethanol) and waste sunflower oil was studied. Materials and methods. Strain IMV B-7005 was grown in liquid mineral media, containing the mixture of С2-С6-substrates and sunflower oil of various quality (refined, waste oil after frying potatoes, meat, vegetables, or mixed one after frying various foods). The EPS concentration was determined gravimetrically after precipitation with isopropanol, the EPS-synthesizing ability - as the ratio of the EPS concentration to the concentration of biomass and expressed in g EPS/g biomass. Results and discussion. After replacement of refined sunflower oil in the mixture with molasses on various types of waste one (after frying potatoes, meat, vegetables, or mixed oil after frying various foods) the concentration of synthesized ethapolan was 10- 14 g/l. Further neutralization of the hydrolyzed molasses with a simultaneous increase in the concentration of monosubstrates in the mixture to 3.0% was accompanied by an increase in the amount of synthesized EPS to 15.3-16.3 g/l, which remained at the same level regardless of the presence in the mixed substrate of different batches of mixed waste oil. Maximum indicators of ethapolan synthesis (15.6-15.9 g/l) on the mixture of ethanol and waste oil were achieved in the case of increasing in the culture medium content of magnesium cations (activator of key enzyme of C2-metabolism in the producer of ethapolan) from 1.5 to 5 mM and fractional application of substrates (4 or 5 portions). Replacement of the refined substrate on mixed waste oil after frying various foods in the mixture with sodium acetate was accompanied by a slight decrease in the amount of synthesized ethapolan (from 17.3 to 16.4 g/l). However, EPS-synthesizing ability reached 7.3 g EPS/ g biomass, which was 3.7 and 2.2 times higher than the indicators obtained during the cultivation of ethapolan producer on the mixture of mixed waste oil with molasses and ethanol, respectively. Conclusions. The obtained results are the basis for the development of universal technology for the ethapolan synthesis on mixed substrates, independent of the supplier and the quality of waste sunflower oil.
- Subjects
PETROLEUM waste; SUNFLOWER seed oil; FRENCH fries; MICROBIAL exopolysaccharides; ACINETOBACTER; MICROBIOLOGICAL synthesis; ISOPROPYL alcohol
- Publication
Ukrainian Journal of Food Science, 2021, Vol 9, Issue 2, p259
- ISSN
2310-1008
- Publication type
Article
- DOI
10.24263/2310-1008-2021-9-2-12