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- Title
Dynamic nano-imaging of label-free living cells using electron beam excitation-assisted optical microscope.
- Authors
Fukuta, Masahiro; Kanamori, Satoshi; Furukawa, Taichi; Nawa, Yasunori; Inami, Wataru; Lin, Sheng; Kawata, Yoshimasa; Terakawa, Susumu
- Abstract
Optical microscopes are effective tools for cellular function analysis because biological cells can be observed non-destructively and non-invasively in the living state in either water or atmosphere condition. Label-free optical imaging technique such as phase-contrast microscopy has been analysed many cellular functions, and it is essential technology for bioscience field. However, the diffraction limit of light makes it is difficult to image nano-structures in a label-free living cell, for example the endoplasmic reticulum, the Golgi body and the localization of proteins. Here we demonstrate the dynamic imaging of a label-free cell with high spatial resolution by using an electron beam excitation-assisted optical (EXA) microscope. We observed the dynamic movement of the nucleus and nano-scale granules in living cells with better than 100 nm spatial resolution and a signal-to-noise ratio (SNR) around 10. Our results contribute to the development of cellular function analysis and open up new bioscience applications.
- Subjects
CELL physiology; ELECTRON beams; OPTICAL microscopes; PHASE-contrast microscopy; ENDOPLASMIC reticulum; GOLGI apparatus; SIGNAL-to-noise ratio
- Publication
Scientific Reports, 2015, p16068
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep16068