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- Title
Novel (S)-Selective Hydrolase from Arthrobacter sp. K5 for Kinetic Resolution of Cyclic Amines.
- Authors
Fukawa, Yuta; Mizuno, Yuta; Kawade, Keisuke; Mitsukura, Koichi; Yoshida, Toyokazu
- Abstract
Chiral 2-methylpiperidine (2-MPI) is an important building block that has potential for applications in pharmaceuticals and pesticides. In this study, we observed that the hydrolase in Arthrobacter sp. K5 exhibits high (S)-selectivity toward rac-N-pivaloyl-2-MPI to yield (S)-2-MPI with 80.2% enantiomeric excess (ee) in a 38.2% conversion. The hydrolase, which was identified by analyses of partial amino acid sequences of the purified enzyme and genome sequence of Arthrobacter sp. K5, exhibited moderate homology with amidohydrolases up to 67% (molinate hydrolase from Gulosibacter molinativorax). The hydrolase gene was overexpressed in Rhodococcus erythropolis. The recombinant cells produced (S)-2-MPI with 83.5% ee in a 48.4% conversion (E = 26.3) from 100 mM rac-N-pivaloyl-2-MPI. These results suggest the possibility of an efficient preparation of chiral 2-MPI in kinetic resolution.
- Subjects
KINETIC resolution; AMINO acid sequence; ARTHROBACTER; RHODOCOCCUS erythropolis; AMINES; AMIDASES
- Publication
Catalysts (2073-4344), 2021, Vol 11, Issue 7, p809
- ISSN
2073-4344
- Publication type
Article
- DOI
10.3390/catal11070809